| Formula | (g/l) |
| Enzymatic Digest of Casein | 5 |
| Enzymatic Digest of Animal Tissue | 5 |
| Beef Extract | 1 |
| D-Mannitol | 10 |
| Sodium Chloride | 75 |
| Phenol Red | 0.025 |
| Agar | 15 |
| Final pH: | 7.4 ± 0.2 at 25˚C |